CPT tube 8ml
A Vcutainer CPT (Cell Preparation Tube) 8mL bututu ne na musamman na tarin jini da ake amfani da shi don keɓe ƙwayoyin mononuclear (PBMCs) daga dukan jini. Ana amfani da shi a cikin ilimin rigakafi, bincike na cututtuka, da aikace-aikacen asibiti kamar kulawar rigakafi da ƙididdigar tushen tantanin halitta.
Mahimman Fasalolin Tube CPT (8mL):
An riga an cika shi da maganin maƙarƙashiya da matsakaicin gradient mai yawa
Ya ƙunshi Sodium Citrate a matsayin maganin rigakafi.
Ya ƙunshi Ficoll™ tushen yawa gradient separation gel don sauƙaƙe rarrabuwar ƙwayoyin mononuclear.
Yana ba da damar keɓewar PBMC mataki ɗaya
Yana kawar da buƙatu na al'ada mai yawa gradient centrifugation (misali, Ficoll-Paque™).
Yana rage lokacin sarrafawa da canzawa.
Daidaitaccen ƙarfin ƙarar jini
An tsara shi don sarrafa har zuwa 8mL na cikakken jini.
Bakararre, bututu mai amfani guda ɗaya
Ana amfani da shi don aikace-aikacen asibiti da bincike inda ake buƙatar keɓewar PBMC mai inganci.
Mai jituwa tare da centrifugation
Ana buƙatar takamaiman ƙa'idar centrifugation don samun nasarar raba PBMCs daga sauran sassan jini.
Amfani da Aikace-aikace:
PBMC keɓancewa don nazarin aikin rigakafi (kwayoyin T, ƙwayoyin B, ƙwayoyin NK, monocytes).
Sitometry mai gudana, ELISPOT, cytokine assays, da sauran nazarin rigakafi.
Cutar cututtuka da bincike na rigakafi.
Kula da cututtuka na autoimmune.
Gwaje-gwajen asibiti da gano biomarker.
Yadda Yake Aiki (Tsarin Farko):
1. Tattara samfurin jini a cikin bututun CPT (cika zuwa ƙarar da aka zaɓa).
2. Juya a hankali (sau 8-10) don haɗuwa tare da maganin ƙwanƙwasa.
3. Centrifuge kamar yadda ka'idar masana'anta (yawanci a kusa da 1500-1800 RCF na minti 20-30 a dakin da zafin jiki).
4. PBMC Layer siffofi sama da gel SEPARATOR bayan centrifugation.
5. A hankali cire Layer PBMC ta amfani da pipette don aikace-aikacen ƙasa.
Adana da Gudanarwa:
Ajiye a dakin da zafin jiki (15-25 ° C).
Guji zafi mai yawa ko daskarewa.
